Immunological Sciences

Anti-tdTomato Goat Polylclonal Antibody

Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 μg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.

Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 μg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.

Catalog Number:AB-84822
Conjugate:Unconjated
Size:600 ug
Concentration:3.00 mg/ml
Host:Goat
Isotype:IgG
Clone:POLY
Immunogen:Purified recombinant peptide produced in E. coli.
Reactivity:Transfected Cells
Applications:Western Blot:    1:500-1:2,000 Immunohistochemistry (frozen tissues):  :1:50-1:500 Immunohistochemistry (paraffin-embedded tissues):  :1:50-1:500 Immunofluorescence:  :1:50-1:500

Applications

Western Blot:    1:500-1:2,000 Immunohistochemistry (frozen tissues):  :1:50-1:500 Immunohistochemistry (paraffin-embedded tissues):  :1:50-1:500 Immunofluorescence:  :1:50-1:500

Immunogen

Purified recombinant peptide produced in E. coli.

Target Background

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research. <dl class="mb-10"> <dt class="text-sm leading-5 font-medium text-gray-500"></dt> </dl> &nbsp;

Storage

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Buffer

​Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 μg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.

Immunofluorescence – anti-tdTomato Ab in 293HEK cells transfected with cDNA at 1/50 dilution; cells were fixed with 4% of PFA.

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