Immunological Sciences

α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody

Western blot analysis of Mouse heart, using α-Smooth Muscle Actin (ACTA2) antibody at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of Mouse heart, using α-Smooth Muscle Actin (ACTA2) antibody at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-84750
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human α-Smooth Muscle Actin (ACTA2).
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:42KDa
Purification:Affinity purification
Form:Liquid
Synonyms:ACTSA

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues) 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human α-Smooth Muscle Actin (ACTA2).

Target Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.

Synonyms:ACTSA

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of Mouse heart, using α-Smooth Muscle Actin (ACTA2) antibody at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Immunofluorescence analysis of human smooth muscle cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse colon cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat rectum cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffinembedded human colon carcinoma using α-Smooth Muscle Actin Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded human esophagus using α- Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded mouse lung using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded mouse stomach using α- Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded Rat heart using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded rat ovary using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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