Immunological Sciences

PBEF Rabbit Polyclonal Antibody

Western Blot analysis of 1,mouse-lung 2,mouse-kidneycells using primary antibody diluted at 1:500(4°Covernight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Western Blot analysis of 1,mouse-lung 2,mouse-kidneycells using primary antibody diluted at 1:500(4°Covernight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Catalog Number:AB-E12102
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human PBEF. at AA range: 411-460
Reactivity:Human;Mouse;Rat
Applications:Western Blot:    1:500-2000 Immunohistochemistry (paraffin-embedded tissues):  1:50-300
Molecular Weight:54kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin

Applications

Western Blot:    1:500-2000 Immunohistochemistry (paraffin-embedded tissues):  1:50-300

Immunogen

Synthesized peptide derived from human PBEF. at AA range: 411-460

Target Background

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yieldnicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide.The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Synonyms:Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of 1,mouse-lung 2,mouse-kidneycells using primary antibody diluted at 1:500(4°Covernight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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