Immunological Sciences

ACCalpha Rabbit Polyclonal Antibody

Western Blot analysis of 3T3 cells using ACCα Polyclonal Antibody

Western Blot analysis of 3T3 cells using ACCα Polyclonal Antibody

Catalog Number:AB-E3452
Conjugate:Unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Acetyl-CoA Carboxylase. AA range:46-95.
Reactivity:Human;Mouse;Rat;Bovine;Canine
Applications:Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300
Molecular Weight:265kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
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Applications

Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human Acetyl-CoA Carboxylase. AA range:46-95.

Target Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternativelyspliced transcript variants divergent in the 5'sequence and encoding distinct isoforms have beenfound for this gene.

Synonyms:ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of 3T3 cells using ACCα Polyclonal Antibody

Western Blot analysis of NIH-3T3 cells using ACCα Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorb

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Acetyl-CoA Carboxylase Antibody. The picture on the right is blocked with the synthesized peptide.

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