Actin beta HRP

Western blot analysis of extracts of various cell lines, using HRP-Conjugated β-actin antibody  at 1:50000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using HRP-Conjugated β-actin antibody at 1:50000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 3s.

Catalog Number:MAB-24008HRP
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG1
Clone:AC.15
Immunogen:Recombinant protein of human β-actin
Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryzasativa
Applications:Western Blot: 1:5000 - 1:50000
Molecular Weight:43kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:5000 - 1:50000

Immunogen

Recombinant protein of human β-actin

Target Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using HRP-Conjugated β-actin antibody at 1:50000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 3s.

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