Immunological Sciences

Adipsin Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using Adipsin antibody  at1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL West Pico Plus._Exposure time: 20s.

Western blot analysis of extracts of various cell lines, using Adipsin antibody  at1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL West Pico Plus._Exposure time: 20s.

Catalog Number:AB-84821
Conjugate:Unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human Adipsin.
Reactivity:Human,Mouse
Applications:Western Blot:    1:500 - 1:2000
Molecular Weight:27kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:DF; ADN; PFD; ADIPSIN; CFD

Applications

Western Blot:    1:500 - 1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human Adipsin.

Target Background

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases.This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.

Synonyms:DF; ADN; PFD; ADIPSIN; CFD

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Adipsin antibody  at1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL West Pico Plus._Exposure time: 20s.

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