AF10 Rabbit Monoclonal Antibody

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Catalog Number:MABN01619
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:Recombinant protein of human AF10
Reactivity:Human
Applications:WB 1:500-1:1000,IHC 1:50-1:100
Molecular Weight:Calculated MW: 113 kDa; Observed MW: 140 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein

Applications

WB 1:500-1:1000,IHC 1:50-1:100

Immunogen

Recombinant protein of human AF10

Target Background

Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (PubMed:26439302).

Synonyms:MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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