AFP (1J18) Rabbit Monoclonal Antibody

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Catalog Number:MABN06665
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human alpha 1 Fetoprotein
Reactivity:Human
Applications:WB 1:500-1:2000,IHC 1:50-1:200,IP 1:20-1:50,IF-P 1:50-1:200
Molecular Weight:69kDa
Purification:Affinity purification
Form:Liquid
Synonyms:Alpha fetoglobulin; Alpha fetoprotein precursor; FETA; HPAFP;

Applications

WB 1:500-1:2000,IHC 1:50-1:200,IP 1:20-1:50,IF-P 1:50-1:200

Immunogen

A synthetic peptide of human alpha 1 Fetoprotein

Target Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma . Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Synonyms:Alpha fetoglobulin; Alpha fetoprotein precursor; FETA; HPAFP;

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

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