AKT Rabbit Monoclonal Antibody

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Catalog Number:MABN03759
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Reactivity:Human,Mouse,Rat,Hamster
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,IP 1:20-1:50
Molecular Weight:Calculated MW: 56 kDa; Observed MW: 56 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:AKT1

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,IP 1:20-1:50

Target Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Synonyms:AKT1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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