Immunological Sciences

AKT1

Anti – Akt (AB­82322) Western blot analysis of Akt1 protein in mouse crude protein brain extract: lane 2 – 20 µg; lane 3 -100 µg; lane 4 - 200 µg of total protein loaded. Lane 1 represents the band corresponding to a 100ng of recombinant human Akt1

Anti – Akt (AB­82322) Western blot analysis of Akt1 protein in mouse crude protein brain extract: lane 2 – 20 µg; lane 3 -100 µg; lane 4 - 200 µg of total protein loaded. Lane 1 represents the band corresponding to a 100ng of recombinant human Akt1

Catalog Number:AB-82322
Size:100 ul
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Peptide derived from the C-terminal sequence of human AKT1 protein
Reactivity:Hu, Ms, Rt
Applications:Western blotting –1:1,000 - ELISA – 1:100,000 – 1:200,000
Purification:Purified
Form:Liquid

Applications

Western blotting –1:1,000 - ELISA – 1:100,000 – 1:200,000

Immunogen

Peptide derived from the C-terminal sequence of human AKT1 protein

Target Background

Major clone of IgG obtained from the crude rabbit antiserum by in vitro cloning technology, detecting the Akt1 protein. This antibody cross-reacts with the Akt2 and Akt3 proteins at the lower signal intensity.

Storage

10 µl aliquots at -20 °C.

Buffer

20 mM Tris-HCl, pH 8.0

Anti – Akt (AB­82322) Western blot analysis of Akt1 protein in mouse crude protein brain extract: lane 2 – 20 µg; lane 3 -100 µg; lane 4 - 200 µg of total protein loaded. Lane 1 represents the band corresponding to a 100ng of recombinant human Akt1

Immunoprecipitation-western blot analysis of Akt1 from mouse brain tissue 1. Recombinant Akt1 (50ng; positive control). 2. 200 µg of mouse brain protein extract, immuprecipitation protocol followed without the primary antibody (negative control). Akt1 kinase immunoprecipitation from 200 µg of mouse brain protein extract with Akt1 antibody

No documents available.