Immunological Sciences

Alexa Fluor 405, Goat Anti Mouse IgG (H+L)

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Catalog Number:IS20080
Size:1 mg
Concentration:2mg/ml
Host:Goat
Isotype:IgG
Clone:POLY
Reactivity:Mouse
Applications:Fluorescence microscopy: 1-2 ug/mL FC: 1 ug/106 cells Near-infrared western detection: 50-100 ng/mL
Purification:Aff. Pur.
Form:liquid

Spectral Properties λAbs/λEm = 408/452 nm (in pH 7.4 PBS buffer) ALEXA FLUOR 405 is spectrally similar to Pacific Blue®.

Applications

Fluorescence microscopy: 1-2 ug/mL FC: 1 ug/106 cells Near-infrared western detection: 50-100 ng/mL

Target Background

ALEXA FLUOR 405 antibodies are affinity-purified antibodies labeled with a blue fluorescent dye Alexa Fluor 405, one of an outstanding series of Alexa Fluor dyes developed by IS. Alexa Fluor dyes are superior to other commercially available dyes for antibody labeling by having combined advantages in brightness, photostability, and specificity

Storage

Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.

Buffer

pH~7.4 PBS containing 50% glycerol, 2 mg/ml bovine serum albumin (IgG-free and protease-free) and 0.05% sodium azide.

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