Immunological Sciences

Alexa Fluor 633, Donkey anti-chicken IgY(H+L) Highly cross-ads.

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Catalog Number:IS20168
Size:1 mg
Concentration:2mg/ml
Host:Donkey
Isotype:IgG
Clone:POLY
Reactivity:Chicken
Applications:Recommended Dilution Range 1-10 µg/mL of the IgG conjugate for most applications. Appropriate dilutions of the conjugate should be determined empirically.
Purification:Aff. Pur.
Form:liquid

Color and Form: Blue solution. Spectral Properties λabs/λem = 630/650 nm (in pH 7.4 PBS buffer) Alexa Fluor 633 is spectrally similar to DyLightTM 633.

Applications

Recommended Dilution Range 1-10 µg/mL of the IgG conjugate for most applications. Appropriate dilutions of the conjugate should be determined empirically.

Target Background

Far-red fluorescent dyes offer the advantage of ultra sensitive detection because background signal due to auto-fluorescence in most biological samples is minimal in this spectral region. For many years, the cyanine dye Cy-5 has been the dye of choice for such detection. More recently, Alexa Fluor® 647 has been developed as a better alternative by having brighter fluorescence and higher photostability. On the other hand, while Alexa Fluor® 633 is photostable, its fluorescence on proteins is very weak

Storage

Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.

Buffer

In PBS pH ~7.4 containing 50% glycerol, 2 mg/mL bovine serum albumin (IgG-free and protease-free) and 0.05% sodium azide.

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