Immunological Sciences

Alexa Fluor 647, Goat anti Chicken IgY (H+L) Highly cross-ads.

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Catalog Number:IS20044
Size:1 mg
Concentration:2mg/ml
Host:Goat
Isotype:IgG
Clone:POLY
Reactivity:Chicken
Applications:Recommended Dilution Range Fluorescence microscopy: 1-2 ug/mL Flow cytometry: 1 ug/106 cells Near-infrared western detection: 50-100 ng/m
Purification:Aff. Pur.
Form:liquid

Color and Form: Blue solution.Spectral Properties:λabs/λem = 650/665 nm (in pH 7.4 PBS buffer)ALEXA FLUOR647 is spectrally similar to CyTM5, Alexa Fluor® 647and DyLightTM 649

Applications

Recommended Dilution Range Fluorescence microscopy: 1-2 ug/mL Flow cytometry: 1 ug/106 cells Near-infrared western detection: 50-100 ng/m

Target Background

Alexa Fluor 647 antibodies are affinity-purified antibodies labeled with a red fluorescent dye ALEXA FLUOR647, one of an outstanding series of ALEXA FLUOR dyes developed by Immunological Sciences. ALEXA FLUOR dyes are superior to both Alexa® Fluor dyes and CyTM dyes for antibody labeling by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging (Please visit our website for details).

Storage

Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.

Buffer

2 mg/mL in pH~7.4 PBS containing 50% glycerol, 2 mg/ml bovine serum albumin (IgG-free and protease-free) and 0.05% sodium azide.

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