AMBRA1

Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-83947
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human AMBRA1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 -1:100
Molecular Weight:143kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:AMBRA1; DCAF3; WDR94; autophagy and beclin 1 regulator 1

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 -1:100 Immunofluorescence: 1:50 -1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human AMBRA1

Target Background

Synonyms:AMBRA1; DCAF3; WDR94; autophagy and beclin 1 regulator 1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using AMBRA1 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse stomach using AMBRA1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using AMBRA1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using AMBRA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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