Immunological Sciences

Androgen Receptor Rabbit Polyclonal Antibody

Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000.

Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000.

Catalog Number:AB-91391
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from the Internal region of human AR.
Reactivity:Human, Rat, Mouse
Applications:Western Blot:   1:500-2000 Immunofluorescence:  1:50-200 Immunohistochemistry (paraffin-embedded tissues)1:50-300 ELISA 1:10000-20000
Molecular Weight:99kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:AR; DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4

Applications

Western Blot:   1:500-2000 Immunofluorescence:  1:50-200 Immunohistochemistry (paraffin-embedded tissues)1:50-300 ELISA 1:10000-20000

Immunogen

Synthesized peptide derived from the Internal region of human AR.

Target Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62  repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoform.

Synonyms:AR; DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000.

Immunofluorescence analysis of human-stomach tissue.1,AR Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B:  DAPI. Picture C:  merge of A+B

Immunofluorescence analysis of human-stomach tissue. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.

Immunofluorescence analysis of rat-heart tissue. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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