Immunological Sciences

Angiopoietin 1

Western blot analysis of extracts of various cell lines, using ANGPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL West Pico Plus Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using ANGPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL West Pico Plus Exposure time: 90s.

Catalog Number:AB-82336
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human ANGPT1
Reactivity:Hu, Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100
Molecular Weight:51kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ANGPT1;AGP1;AGPT;ANG1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human ANGPT1

Target Background

This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cellspecific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms:ANGPT1;AGP1;AGPT;ANG1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using ANGPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL West Pico Plus Exposure time: 90s.

Immunohistochemistry of paraffin embedded rat lung using ANGPT1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse lung using ANGPT1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded human esophagus using ANGPT1 antibody at dilution of 1:100 (40x lens).

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