Annexin VI

Western blot analysis of extracts of
various cell lines, using ANXA11
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus .
Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using ANXA11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus . Exposure time: 30s.

Catalog Number:AB-84193
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11
Reactivity:Hu, Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:56kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ANXA11; ANX11; CAP50; annexin A11

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11

Target Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.

Synonyms:ANXA11; ANX11; CAP50; annexin A11

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using ANXA11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus . Exposure time: 30s.

Immunofluorescence analysis of A549 cells using ANXA11 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody. Western blot was performed from the immunoprecipitate using ANXA11 antibody at a dilition of 1:1000.

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