ANT2

Western blot analysis of extracts of various cell lines, using SLC25A6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using SLC25A6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Catalog Number:AB-84386
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human SLC25A6.
Reactivity:Hu, Ms, Rt
Applications:WB: 1:200-1:2000
Molecular Weight:33kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SLC25A6;AAC3;ANT;ANT 2;ANT 3;ANT3;ANT3Y

Applications

WB: 1:200-1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human SLC25A6.

Target Background

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein is implicated in the function of the permability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis. The human genome contains several non-transcribed pseudogenes of this gene.

Synonyms:SLC25A6;AAC3;ANT;ANT 2;ANT 3;ANT3;ANT3Y

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using SLC25A6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Immunofluorescence analysis of NIH/3T3 cells using SLC25A6 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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