Anti-AMPK alpha 1 antibody (Rabbit mAb)
WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
| Catalog Number: | ABS150024 |
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,κ |
| Immunogen: | Recombinant protein corresponding to Human AMPK alpha 1 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot:1: 1000-1:2000 Immunoistochemistry (paraffin emebbed tissues) 1:500 - Immunofluorescence 1:300 |
| Molecular Weight: | 64 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | None |
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Applications
Western Blot:1: 1000-1:2000 Immunoistochemistry (paraffin emebbed tissues) 1:500 - Immunofluorescence 1:300
Immunogen
Recombinant protein corresponding to Human AMPK alpha 1
