Anti-Beclin 1 Rabbit pAb
WB analysis of Beclin 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
| Catalog Number: | ABS115741 |
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to Human Beclin 1 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB:1: 500-1: 1000 IHC (paraffin Embedded tissues) :1:500 IF : 1:300 |
| Molecular Weight: | 52 kDa / 60 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer.
Applications
WB:1: 500-1: 1000 IHC (paraffin Embedded tissues) :1:500 IF : 1:300
Immunogen
Recombinant protein corresponding to Human Beclin 1
