Anti-beta Actin Rabbit Polyclonal Antibody

WB analysis of beta Actin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:ABS11001
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin
Reactivity:Species, Human, Mouse, Rat
Applications:WB 1: 1000-1: 2000<br/>IHC/IF 1: 500-1: 1000<br/>ICC/IF 1: 300-1: 1000
Molecular Weight:42 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Applications

WB 1: 1000-1: 2000<br/>IHC/IF 1: 500-1: 1000<br/>ICC/IF 1: 300-1: 1000

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin

Target Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Synonyms:ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of beta Actin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

IF analysis ofbeta Actinred.Sample: Human colon cancer, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

IF analysis ofbeta Actinred.Sample: Rat liver, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

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