Immunological Sciences

Anti-Calbindin Rabbit Monoclonal Antibody

WB analysis of Calbindin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of Calbindin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:MAB-94138
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:4H7
Immunogen:Recombinant protein corresponding to Mouse Calbindin
Reactivity:Species, Mouse, Rat, Human, Mouse, Rat
Applications:Applications Dilution<br/>WB 1: 500-1: 1000<br/>IHC/IF 1: 1000-1: 3000
Purification:Affinity purification
Form:Liquid
Synonyms:Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1, CAB27, CALB1, CALB, Calbindin 1

Calbindin 1 is a protein that in humans is encoded by the CALB1 gene.  The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.

Applications

Applications Dilution<br/>WB 1: 500-1: 1000<br/>IHC/IF 1: 1000-1: 3000

Immunogen

Recombinant protein corresponding to Mouse Calbindin

Target Background

Calbindin 1 is a protein that in humans is encoded by the CALB1 gene.  The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.

Synonyms:Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1, CAB27, CALB1, CALB, Calbindin 1

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of Calbindin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

IHC analysis of Calbindin.Sample: Human renal cancer, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of Calbindin.Sample: Mouse brain, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of Calbindin.Sample: Mouse kidney, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of Calbindin.Sample: Rat brain, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of Calbindin.Sample: Rat kidney, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IF analysis of Calbindinred.Sample: Mouse kidney, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 2000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

IF analysis of Calbindinred.Sample: Mouse small intestine, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 2000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

IF analysis of Calbindinred.Sample: Rat brain, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 2000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

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