Anti-Ki67 Mouse mAb

Product image
Catalog Number:ABS121141
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG1,κ
Immunogen:KLH conjugated Synthetic peptidecorresponding to Human Ki67
Reactivity:Human, Mouse, Rat
Applications:ICC/IF<br/>1: 100-1: 500
Purification:Affinity purification
Form:Liquid

equired to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility . Images

Applications

ICC/IF<br/>1: 100-1: 500

Immunogen

KLH conjugated Synthetic peptidecorresponding to Human Ki67

Target Background

equired to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility . Images

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Ki67 at dilution of 1: 600

Immunohistochemistry analysis of paraffin-embedded mouse testis using Ki67 at dilution of 1: 600

Immunohistochemistry analysis of paraffin-embedded rat testis using Ki67 at dilution of 1: 600

Immunohistochemistry analysis of paraffin-embedded rat colon using Ki67 at dilution of 1: 600

Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Ki67 at dilution of 1: 600

Immunofluorescent analysis of paraformaldehyde-fixed rat testis using Ki67 at dilution of 1: 600

ICC analysis of Ki67red. Sample: HeLa, 4% PFA 20min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.

ICC analysis of Ki67red. Sample: NIH-3T3, 4% PFA 20min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.

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