Immunological Sciences

Anti-Ki67 Rabbit pAb

IHC analysis of ki67 (GB111141).<br/>Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 1000, 4℃ overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of ki67 (GB111141).<br/>Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 1000, 4℃ overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

Catalog Number:ABS111141
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse Ki67
Reactivity:Mouse, Rat
Applications:IHC/IF<br/>1: 500-1: 1200
Purification:Affinity purification

Applications

IHC/IF<br/>1: 500-1: 1200

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Ki67

Target Background

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.

Buffer

PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

IHC analysis of ki67 (GB111141).<br/>Sample: Mouse placenta (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 1000, 4℃ overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of Ki67 (GB111141).<br/>Sample: Mouse subcutaneous transplant tumor（H22） (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 500, 4℃ overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of ki67 (GB111141).<br/>Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 750, 4℃ overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IF analysis of ki67 (GB111141).<br/>Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 600, 4℃ overnight.<br/>Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

IF analysis of ki67 (GB111141).<br/>Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.<br/>Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.<br/>Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.<br/>Primary antibody: 1: 600, 4℃ overnight.<br/>Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

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