Anti-PCNA Rabbit pAb

Product image
Catalog Number:ABS11010
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Reactivity:Human, Mouse, Rat
Applications:IHC/IF<br/>1: 200-1: 1000
Purification:Affinity purification
Form:Liquid

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. Images

Applications

IHC/IF<br/>1: 200-1: 1000

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Target Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. Images

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.

Buffer

PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

Immunofluorescence of paraffin embedded rat testis using PCNA at dilution of 1: 500

ICC analysis of PCNAred. Sample: HeLa, 4% PFA 20min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

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