Immunological Sciences

Anti-Tyrosine Hydroxylase Rabbit Polyclonal Antibody

WB analysis of Tyrosine Hydroxylase . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of Tyrosine Hydroxylase . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

Catalog Number:ABS11181
Conjugate:Unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein corresponding to Mouse Tyrosine Hydroxylase
Reactivity:Human, Mouse, Rat, Human, Mouse, Rat, Monkey
Applications:Western Blot: 1: 500-1: 1000 Immunohistochemistry: 1: 500-1: 2000 Immunofluorescence: 1: 500-1: 2000
Molecular Weight:62 kDa / 62 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:TH, Th, DYT14, DYT5b, TYH, tyrosine hydroxylase, Tyrosine hydroxylase

Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). It does so using molecular oxygen(O2), as well as iron (Fe2+) and tetrahydrobiopterin as cofactors. L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine(adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines.

Applications

Western Blot: 1: 500-1: 1000 Immunohistochemistry: 1: 500-1: 2000 Immunofluorescence: 1: 500-1: 2000

Immunogen

Recombinant protein corresponding to Mouse Tyrosine Hydroxylase

Target Background

Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). It does so using molecular oxygen(O2), as well as iron (Fe2+) and tetrahydrobiopterin as cofactors. L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine(adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines.

Synonyms:TH, Th, DYT14, DYT5b, TYH, tyrosine hydroxylase, Tyrosine hydroxylase

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.

Buffer

PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

WB analysis of Tyrosine Hydroxylase . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse corpus striatum , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse substantia nigra , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Rat corpus striatum , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Rat substantia nigra , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IF analysis of TH . Sample: Mouse corpus striatum , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300 RT, 1h.

IF analysis of TH . Sample: Mouse substantia nigra , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300 RT, 1h.

IF analysis of TH . Sample: Rat corpus striatum , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300 RT, 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Monkey brain , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse striatum, 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse striatum, 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse substantia nigra, 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

IHC analysis of Tyrosine Hydroxylase . Sample: Mouse substantia nigra, 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

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