Anti-Vimentin Rabbit Polyclonal Antibody

WB analysis of Vimentin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of Vimentin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

Catalog Number:ABS111308
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:KLH conjugated Synthetic peptidecorresponding to Mouse Vimentin
Reactivity:Human,Mouse,Rat
Applications:WB<br/>1: 500-1: 1000 IHC/IF: 1: 1500-1: 3000
Molecular Weight:54 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:VIM, CTRCT30, HEL113, vimentin

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions.

Applications

WB<br/>1: 500-1: 1000 IHC/IF: 1: 1500-1: 3000

Immunogen

KLH conjugated Synthetic peptidecorresponding to Mouse Vimentin

Target Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions.

Synonyms:VIM, CTRCT30, HEL113, vimentin

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.

Buffer

PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

WB analysis of Vimentin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of Vimentin . Sample: Human breast cancer , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of Vimentin . Sample: Human breast cancer , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

No documents available.