APAF1 Mouse Monoclonal Antibody

No images available

Catalog Number:MABN82023
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG2b
Clone:Monoclonal
Immunogen:Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
Reactivity:Human
Applications:ELISA 1:5000-1:20000,FC 1:200-1:400
Molecular Weight:141.8kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:CED4; APAF-1

Applications

ELISA 1:5000-1:20000,FC 1:200-1:400

Immunogen

Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.

Target Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Synonyms:CED4; APAF-1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide

No documents available.