Immunological Sciences

APAF1 Rabbit Monoclonal Antibody

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Catalog Number:MABN87003
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:Recombinant protein of human APAF1
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:2000,IP 1:20-1:50
Molecular Weight:Calculated MW:142 kDa; Observed MW:142 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:CED4; APAF-1

Applications

WB 1:500-1:2000,IP 1:20-1:50

Immunogen

Recombinant protein of human APAF1

Target Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Synonyms:CED4; APAF-1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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