Immunological Sciences

Apelin Rabbit Polyclonal Antibody

Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Catalog Number:AB-E3923
Conjugate:Unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesizedpeptide derived from the C-terminal region of human APLN. AA range:28-77
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000Immunohisothcemistry(paraffin-embedded tissues): 1:100-300
Molecular Weight:16kD
Purification:The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form:Liquid
Synonyms:APLN; APEL; Apelin; APJ endogenous ligand
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Applications

Western Blot: 1/500 - 1/2000Immunohisothcemistry(paraffin-embedded tissues): 1:100-300

Immunogen

The antiserum was produced against synthesizedpeptide derived from the C-terminal region of human APLN. AA range:28-77

Target Background

This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signalingpathways that regulate diverse biological functions including fluid homeostasis, cardiovascular functionand insulin secretion. This protein also functions as acoreceptor for the human immunodeficiency virus 1.

Synonyms:APLN; APEL; Apelin; APJ endogenous ligand

Storage

Store at -20°C. Avoid repeated freeze-thawcycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide

Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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