Immunological Sciences

APOA1 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using APOA1 antibody at 1:500dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using APOA1 antibody at 1:500dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-84735
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1.
Reactivity:Human, Mouse, Rat
Applications:Western Blot     1:100 - 1:500 Immunohistochemistry:  1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:26kDa
Purification:Affinity purification
Form:Liquid
Synonyms:APOA1;apo(a)

Applications

Western Blot     1:100 - 1:500 Immunohistochemistry:  1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1.

Target Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Synonyms:APOA1;apo(a)

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using APOA1 antibody at 1:500dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunohistochemistry of paraffin-embeddedmouse liver using APOA1 Rabbit pAb  at dilution of 1:20 (40x lens). Perform highpressure antigen retrieval with 10 mM citratebuffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HepG2 cellsusing APOA1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclearstaining.

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