APPBP1 Rabbit Monoclonal Antibody

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Catalog Number:MABN01665
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human APPBP1
Reactivity:Human
Applications:WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight:Calculated MW: 60 kDa; Observed MW: 60 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1

Applications

WB 1:500-1:1000,IP 1:20-1:50

Immunogen

A synthetic peptide of human APPBP1

Target Background

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Synonyms:NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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