Aquaporin 5 (AQP5) Rabbit Polyclonal Antibody

Western blot analysis of various lysates, using AQP5 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  
Exposure time: 60s.

Western blot analysis of various lysates, using AQP5 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-90206
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 188-203 of human AQP5.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:1000 - 1:5000 Immunofluorescence: 1:50 - 1:200 Immunocytcochemistry: 1:50 - 1:200
Molecular Weight:24 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:PPKB; AQP-5

Applications

Western Blot: 1:1000 - 1:5000 Immunofluorescence: 1:50 - 1:200 Immunocytcochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 188-203 of human AQP5.

Target Background

Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13.

Synonyms:PPKB; AQP-5

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.01% thimerosal,50% glycerol,pH7.3.

Western blot analysis of various lysates, using AQP5 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunofluorescence analysis of NIH/3T3 cells using AQP5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using AQP5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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