ASK1 Rabbit Monoclonal Antibody

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Catalog Number:MABN21269
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Clone:Monoclonal
Immunogen:A synthetic peptide of human ASK1
Reactivity:Human,Mouse
Applications:WB 1:1000-1:5000,IHC 1:100-1:400,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200
Molecular Weight:CalcuLated MW:155kD;Observed MW:155kD
Purification:Protein A
Form:Liquid
Synonyms:MAP3K5;ASK1;MAPKKK5;MEKK5;Mitogen-activated protein kinase kinase kinase 5;Apoptosis signal-reguLating kinase 1;ASK-1;MAPK/ERK kinase kinase 5;MEK kinase 5;MEKK 5

Applications

WB 1:1000-1:5000,IHC 1:100-1:400,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200

Immunogen

A synthetic peptide of human ASK1

Target Background

Cell localization:Cytoplasm.Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re

Synonyms:MAP3K5;ASK1;MAPKKK5;MEKK5;Mitogen-activated protein kinase kinase kinase 5;Apoptosis signal-reguLating kinase 1;ASK-1;MAPK/ERK kinase kinase 5;MEK kinase 5;MEKK 5

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

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