Western blot analysis of extracts of THP-1 cells, using ASM antibody at 1:1000 dilution. THP-1 cells were treated by PMA
 (80 nM) for overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 30s.

Western blot analysis of extracts of THP-1 cells, using ASM antibody at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 30s.

Catalog Number:AB-J199
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human SMPD1 / ASM
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100
Molecular Weight:60kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SMPD1;ASM;ASMASE;NPD;SMPD1/ASM

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100

Immunogen

Recombinant protein of human SMPD1 / ASM

Target Background

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann- Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.

Synonyms:SMPD1;ASM;ASMASE;NPD;SMPD1/ASM

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol, pH7.3.

Western blot analysis of extracts of THP-1 cells, using ASM antibody at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using ASM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunofluorescence analysis of NIH-3T3 cells using ASM Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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