ATG3 Rabbit Polyclonal Antibody

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Catalog Number:ABN01343
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:A synthetic peptide of human ATG3
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200
Molecular Weight:Calculated MW: 36 kDa; Observed MW: 40 kDa
Purification:Affinity Chromatography
Form:Liquid
Synonyms:APG3; APG3L; PC3-96; APG3-LIKE

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200

Immunogen

A synthetic peptide of human ATG3

Target Background

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). Catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Synonyms:APG3; APG3L; PC3-96; APG3-LIKE

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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