Immunological Sciences

ATG4A Mouse Monoclonal Antibody

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Catalog Number:MABN85978
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG2b
Clone:Monoclonal
Immunogen:This ATG4A antibody is generated from a mouse immunized with a recombinant protein.
Reactivity:Human
Applications:WB 1:500-1:1000,IHC 1:100-1:500,ICC 1:25-1:50,FC 1:25-1:50
Molecular Weight:45.3kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:Cysteine protease ATG4A, 3422-, AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A, ATG4A, APG4A, AUTL2

Applications

WB 1:500-1:1000,IHC 1:100-1:500,ICC 1:25-1:50,FC 1:25-1:50

Immunogen

This ATG4A antibody is generated from a mouse immunized with a recombinant protein.

Target Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Synonyms:Cysteine protease ATG4A, 3422-, AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A, ATG4A, APG4A, AUTL2

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide.

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