ATG7

Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus.  Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-83978
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:78kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ATG7;APG7-LIKE;APG7L;GSA7;Apg7/Atg7

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7.

Target Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53- dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Synonyms:ATG7;APG7-LIKE;APG7L;GSA7;Apg7/Atg7

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 90s.

Immunohistochemistry of paraffin embedded human breast cancer using ATG7 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse lung using ATG7 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using ATG7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

No documents available.