Immunological Sciences

ATM

Western blot analysis of extracts of H460 cells, using ATM Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Western blot analysis of extracts of H460 cells, using ATM Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-83953
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human ATM
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:1000
Molecular Weight:310kDa
Purification:Aff. Pur
Form:Liquid
Synonyms:ATM; AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO 1; serine-protein kinase ATM

Applications

Western Blot: 1:500 - 1:1000

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human ATM

Target Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive

Synonyms:ATM; AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO 1; serine-protein kinase ATM

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of H460 cells, using ATM Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

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