Immunological Sciences

ATPASE/Na+K+ alpha 1

Western blot analysis of extracts of A-549 cells, using ATPase Na+/K+ antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit. Exposure time: 60s.

Western blot analysis of extracts of A-549 cells, using ATPase Na+/K+ antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit. Exposure time: 60s.

Catalog Number:AB-84189
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP1A1.
Reactivity:Hu, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:115kDa
Purification:Aff, Pur.
Form:Liquid
Synonyms:ATP1A1transporting subunit alpha 1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP1A1.

Target Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:ATP1A1transporting subunit alpha 1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of A-549 cells, using ATPase Na+/K+ antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin embedded rat kidney using ATPase Na+/K+ antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using ATPase Na+/K+ antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

No documents available.