AXIN2

Western blot analysis of extracts of various cell lines, using AXIN2 antibody  at 1:1000  dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using AXIN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Catalog Number:AB-84313
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100
Molecular Weight:93kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:AXIN2;AXIL;ODCRCS;axin-2

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2.

Target Background

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Synonyms:AXIN2;AXIL;ODCRCS;axin-2

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using AXIN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Immunohistochemistry of paraffin embedded rat brain using AXIN2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse spinal cord using AXIN2 antibody at dilution of 1:100 (40x lens).

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