BDNF Rabbit Polyclonal Antibody

WB analysis of BDNF . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of BDNF . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

Catalog Number:ABS11559
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse BDNF
Reactivity:Mouse, Rat
Applications:IHC/IF 1: 100-1: 200
Molecular Weight:18 kDa
Purification:Affinity purification
Form:Liquid

Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. Neurotrophic factors are found in the brain and the periphery. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, the kidneys, saliva, and the prostate. Images

Applications

IHC/IF 1: 100-1: 200

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse BDNF

Target Background

Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. Neurotrophic factors are found in the brain and the periphery. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, the kidneys, saliva, and the prostate. Images

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.

Buffer

PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

WB analysis of BDNF . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of BDNF . Sample: Mouse brain , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of BDNF . Sample: Rat brain , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

IHC analysis of BDNF . Sample: Mouse hippocampus , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.

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