Immunological Sciences

BECLIN 1

Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Catalog Number:AB-82599
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human BECN1
Reactivity:Hu,Ms,Rt
Applications:WB: 1: 500-1:1000 Immunohistochemistry araffin Embedded tissues 1: 100Immunofluorescence: 1:100
Molecular Weight:60 kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ATG6;VPS30;beclin1
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Applications

WB: 1: 500-1:1000 Immunohistochemistry araffin Embedded tissues 1: 100Immunofluorescence: 1:100

Immunogen

Recombinant protein of human BECN1

Target Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Synonyms:ATG6;VPS30;beclin1

Storage

Store at +4°C for short term, at -20° C for 12 months. Avoid Freeze and Thaw Cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human breast cancer using BECN1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using BECN1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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