Immunological Sciences

beta2- Microglobulin

Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-84271
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immufluorescence: 1:50 - 1:200
Molecular Weight:12kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:B2M;IMD43

Applications

Western Blot: 1:500 - 1:2000 Immufluorescence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin

Target Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Synonyms:B2M;IMD43

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using beta 2 Microglobulin antibody Blue: DAPI for nuclear staining.

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