Immunological Sciences

GRP78 / BIP Rabbit Polyclonal Antibody

Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Catalog Number:AB-E8358
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthetic peptide from human protein at AA range: 505-570.
Reactivity:Human,Mouse,Rat,Fish
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry(paraffin-embedded tissues):  1:50-300
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry(paraffin-embedded tissues):  1:50-300

Immunogen

Synthetic peptide from human protein at AA range: 505-570.

Target Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum  (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Synonyms:78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L)  Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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