Immunological Sciences

BRG1 Rabbit Monoclonal Antibody

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Catalog Number:MABN87742
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of mouse BRG1
Reactivity:Human,Mouse,Rat
Applications:WB 1:2000-1:20000,IHC 1:100-1:200,ICC/IF 1:200-1:500,FC 1:100-1:200,IP 1:10-1:100
Molecular Weight:Calculated MW:185 kDa; Observed MW:220 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo

Applications

WB 1:2000-1:20000,IHC 1:100-1:200,ICC/IF 1:200-1:500,FC 1:100-1:200,IP 1:10-1:100

Immunogen

A synthetic peptide of mouse BRG1

Target Background

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).

Synonyms:Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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