Immunological Sciences

C-Myc

Western blot analysis of MYC using anti-MYC antibody in 1) Jurkat cell lysate;(2) K562 cell lysate.. The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody at 1ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL West Pico Plus. A specific band was detected for MYC.

Western blot analysis of MYC using anti-MYC antibody in 1) Jurkat cell lysate;(2) K562 cell lysate.. The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody at 1ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL West Pico Plus. A specific band was detected for MYC.

Catalog Number:MAB-94579
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:MYC
Immunogen:Recombinant protein of human c-Myc
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:20 - 1:50 Flow Cytometry: 1:20 - 1:50
Molecular Weight:49kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MRTL;MYCC;bHLHe39;c-Myc;MYC

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:20 - 1:50 Flow Cytometry: 1:20 - 1:50

Immunogen

Recombinant protein of human c-Myc

Target Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Synonyms:MRTL;MYCC;bHLHe39;c-Myc;MYC

Storage

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Buffer

Store at -20℃. Avoid freeze / thaw cycles

Western blot analysis of MYC using anti-MYC antibody in 1) Jurkat cell lysate;(2) K562 cell lysate.. The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody at 1ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL West Pico Plus. A specific band was detected for MYC.

IHC analysis of MYC using anti-MYC antibody on paraffin-embedded human lung cancer.

Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.

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