Cadherin-E

Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , MCF-7 (5),PC-12 (6) , NIH/3T3 (7) , C6 (8) and COS7 (9) cell lysate.

Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , MCF-7 (5),PC-12 (6) , NIH/3T3 (7) , C6 (8) and COS7 (9) cell lysate.

Catalog Number:MAB-94623
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:MS
Isotype:IgG1
Clone:7H12
Immunogen:Purified recombinant fragment of human E-cadherin expressed in E. Coli.
Reactivity:Hu, Ms, Mk
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunofluorescence: 1:200-1:500 Flow Cytometry: 1:200-1:400 ELISA: 1:10000
Molecular Weight:135kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunofluorescence: 1:200-1:500 Flow Cytometry: 1:200-1:400 ELISA: 1:10000

Immunogen

Purified recombinant fragment of human E-cadherin expressed in E. Coli.

Target Background

Specificity: E-cadherin monoclonal antibody detects endogenous levels of E-cadherin protein.

Storage

Store at-20°C, and avoid repeat freeze-thaw cycles.

Buffer

Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.

Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , MCF-7 (5),PC-12 (6) , NIH/3T3 (7) , C6 (8) and COS7 (9) cell lysate.

Immunohistochemistry analysis of paraffin-embedded gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining using E-cadherin monoclonal antibody.

Flow cytometric analysis of Hela cells using E-cadherin monoclonal antibody (green) and negative control (purple).

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