Cadherin-N

Western blot analysis using N-cadherin monoclonal antibody  against A431 (1) , NIH/3T3 (2) , HeLa (3) , C6 (4) and LNCap (5) cell lysate.

Western blot analysis using N-cadherin monoclonal antibody against A431 (1) , NIH/3T3 (2) , HeLa (3) , C6 (4) and LNCap (5) cell lysate.

Catalog Number:MAB-94622
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:MS
Isotype:IgG1
Clone:5D5
Immunogen:Purified recombinant fragment of human N-cadherin expressed in E. Coli.
Reactivity:Hu, Ms, Rt
Applications:Western Blot 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunofluorescence: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000
Molecular Weight:100kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunofluorescence: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000

Immunogen

Purified recombinant fragment of human N-cadherin expressed in E. Coli.

Target Background

Specificity: N-cadherin monoclonal antibody detects endogenous levels of N-cadherin protein.

Storage

Store at-20°C, and avoid repeat freeze-thaw cycles.

Buffer

Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.

Western blot analysis using N-cadherin monoclonal antibody against A431 (1) , NIH/3T3 (2) , HeLa (3) , C6 (4) and LNCap (5) cell lysate.

Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) , colon cancer (B) , ovarian cancer (C) and mammary cancer (D) with DAB staining using N-cadherin monoclonal antibody.

Immunofluorescence analysis of A431 cells using N-cadherin monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of PC-2 cells using N-cadherin monoclonal antibody (green) and negative control (purple).

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