Calnexin Rabbit Polyclonal Antibody

WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:ABS111369
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse Calnexin
Reactivity:Species, Human, Mouse, Rat, Human, Mouse
Applications:Applications Dilution<br/>WB 1: 500-1: 1000<br/>ICC/IF 1: 50-1: 100
Purification:Affinity purification
Form:Liquid
Synonyms:Canx, CALX, CNX, FLJ26570, IP90, p90, Major histocompatibility complex class I antigen-binding protein p88, CANX

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Applications

Applications Dilution<br/>WB 1: 500-1: 1000<br/>ICC/IF 1: 50-1: 100

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Calnexin

Target Background

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Synonyms:Canx, CALX, CNX, FLJ26570, IP90, p90, Major histocompatibility complex class I antigen-binding protein p88, CANX

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

ICC analysis of Calnexinred.Sample: HeLa, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 50, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

No documents available.